FujiFilm LAS 3OOO User Manual

Specifications and Applications  
Image Capturing Unit (Camera Head and Controller)  
CCD chip  
Number of pixels  
Pixel size  
Fujifilm Super CCD Area Type chip  
3.2M pixels  
10.75 x 10.75µm  
Cooling  
Two-stage thermoelectric module with air circulation  
-30°C (when room is below 28°C)  
Power focusing; remote and preset control  
1/100 second to 30 hrs (2 hrs to 30 hrs to be set manually)  
Four orders of magnitude  
Cooling temperature  
Focusing  
Exposure time  
Dynamic range  
Gradation  
16 bits (65,536)  
Image size  
12.6MB Max.; 49.2KB Min.  
Maximum sample size  
Binning  
25 x 25cm (wide angle lens); 14 x 21cm (Fujinon VRF43LMD lens)  
1 x 2, 2 x 4, 4 x 8, and 8 x 16 pixels  
USB1.1  
Interface  
Super CCD  
Software  
The binning mode of the LAS-3000 allows researchers to select from four  
binning settings to enhance both imaging sensitivity and image resolution.  
Image capture  
Image analysis  
Dimensions and Weights  
Camera head  
Dark Box IV  
Fujifilm Image Reader (Macand Windows®)  
Fujifilm Image Gauge (Mac); Fujifilm MultiGauge (Windows®)  
Remote Controlled  
Sample is placed on a tray  
180 (W) x 170 (H) x 250mm (D)  
510 (W) x 730 (H) x 480mm (D)  
3.4kg  
Chemiluminescence  
49.0kg  
Analyzing Unit  
Operating system  
Set a tray  
Windows® XP or MacOS 9 & X  
Applicable Reagents and/or Samples  
Sample  
Chemiluminescence  
Fluorescence  
CDP-Star®, ECL, ECL Plus, SuperSignal, ImmunoStar, CSPD®  
EtBr (W/UV light), SYBR® Green I & II, SYPRO® Orange,  
GFP, DY-458XL (blue LED)  
Chemiluminescence Mode  
Pro-QDiamond, Cy3, RFP, DY-520XL (green LED), Alexa Fluor 633,  
Cy5, DY-647 (red LED)  
Fluorescence  
Chemifluorescence  
Remote controlled  
focusing  
Chemifluorescence  
Digitization  
*AttoPhos™  
CBB-stained gel, Silver stained gel (Transilluminator)  
NBT/BCIP-stained membrane. MW marker (Epi-illuminator)  
Filter  
blue LED  
green LED  
red LED  
UV trans-  
illuminator  
Intelligent Dark Box IV  
EPI-illuminator for fluorescence  
EPI-illuminator for digitization  
blue LED (470nm), green LED (520nm), red LED (630nm)  
White-light source  
Fluorescence  
Chemifluorescence Mode  
Exposure  
Transilluminator - for digitization of  
stained gels and autoradiographic films  
Digitization  
White LED  
UV transilluminator**  
UV-light source (312nm)  
Five positions  
White light  
epi-illuminator  
Filter turret  
White trans-  
illuminator  
Printers  
Pictrography 3500  
Digitize Mode  
Analysis  
Lens  
Lens  
High-sensitivity lens (FUJINON VRF43LMD)  
Wide-view lens  
F-number  
Focal length  
Focus  
0.85  
F-number  
Focal length  
Focus  
2.0  
43mm  
24mm  
Science Imaging System  
Remote power focusing  
Bayonet  
Manual  
The image analysis process captures images in three modes:  
chemiluminescence, fluorescence/chemifluorescence and digitization.  
Mount  
Mount  
Adapter to Nikon F mount  
*No license is granted for use of AttoPhos™ to detect nucleic acid on a nylon membrane.  
**No license is granted for pre-labeling gel electrophoresis method with the UV transilluminator.  
Fuji Photo Film Co., Ltd. 26-30, Nishiazabu 2-Chome, Minato-ku, Tokyo 106-8620, Japan, Tel: +81-3-3406-2201, Fax: +81-3-3406-2158  
Fujifilm Medical Systems U.S.A., Inc. 419 West Avenue, Stamford, CT 06902, U.S.A., Tel: +1-203-324-2000 ext. 6112 (1-800-431-1850 ext. 6112 in the U.S.), Fax: +1-203-351-4713  
Fuji Photo Film (Europe) GmbH, Heesenstr. 31, 40549 Düsseldorf, Germany, Tel: +49-211-5089-174, Fax: +49-211-5089-139 http://www.fujifilm.de E-mail: [email protected]  
LAS-3OOO  
Specifications and system configuration subject to change for improvement without notice. All other product names mentioned herein are the trademarks of their respective owners.  

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